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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
01/10/2019 |
Actualizado : |
01/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BÁEZ, F.; CAMARGO, A.A.; GASTAL, G.D.A. |
Afiliación : |
FRANCISCO BÁEZ, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; ÁLVARO A. CAMARGO, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Ultrastructural imaging analysis of the zona pellucida surface in bovine oocytes. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Microscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036. |
DOI : |
10.1017/S1431927619000692 |
Idioma : |
Inglés |
Notas : |
Article history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019. |
Contenido : |
Abstract:
The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and
mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes
(COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development. |
Palabras claves : |
BOVINE; IN VITRO MATURATION; PLATAFORMA SALUD ANIMAL; ULTRASTRUCTURE; ZONA PELLUCIDA. |
Asunto categoría : |
-- |
Marc : |
LEADER 02045naa a2200229 a 4500 001 1060255 005 2019-10-01 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1017/S1431927619000692$2DOI 100 1 $aBÁEZ, F. 245 $aUltrastructural imaging analysis of the zona pellucida surface in bovine oocytes.$h[electronic resource] 260 $c2019 500 $aArticle history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019. 520 $aAbstract: The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes (COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development. 653 $aBOVINE 653 $aIN VITRO MATURATION 653 $aPLATAFORMA SALUD ANIMAL 653 $aULTRASTRUCTURE 653 $aZONA PELLUCIDA 700 1 $aCAMARGO, A.A. 700 1 $aGASTAL, G.D.A. 773 $tMicroscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036.
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
14/09/2022 |
Actualizado : |
14/09/2022 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
DE BARBIERI, I.; FERREIRA, G.; RAMOS, Z.; NAVAJAS, E.; CIAPPESONI, G. |
Afiliación : |
LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO ALVARO FERREIRA DE MATTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ZULLY MARGOT RAMOS ALVEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELLY ANA NAVAJAS VALENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Consequences of contrasting feed efficiency as lamb on later ewe performance. [Abstract] |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
In: Annual Meeting of the European Federation of Animal Science (EAAP), 73., Porto, Portugal, 5-8 September 2022. Book of Abstracts, 73., Session 68, Theatre 4, p.674. |
ISBN : |
978-90-8686-937-4; e-ISBN: 978-90-8686-385-3 |
Idioma : |
Inglés |
Notas : |
Conference website: https://eaap2022.org/ |
Contenido : |
The aim of this study was to evaluate the productive and reproductive performance of ewes with contrasting residual feed intake (RFI) measured as lamb in their first year of life (in the frame of H2020 project SMARTER n°772787). |
Palabras claves : |
EWE; Proyecto SMARTER; Residual feed intake (RFI); SHEEP. |
Asunto categoría : |
L01 Ganadería |
Marc : |
LEADER 01008nam a2200217 a 4500 001 1063569 005 2022-09-14 008 2022 bl uuuu u01u1 u #d 100 1 $aDE BARBIERI, I. 245 $aConsequences of contrasting feed efficiency as lamb on later ewe performance. [Abstract]$h[electronic resource] 260 $aIn: Annual Meeting of the European Federation of Animal Science (EAAP), 73., Porto, Portugal, 5-8 September 2022. Book of Abstracts, 73., Session 68, Theatre 4, p.674.$c2022 500 $aConference website: https://eaap2022.org/ 520 $aThe aim of this study was to evaluate the productive and reproductive performance of ewes with contrasting residual feed intake (RFI) measured as lamb in their first year of life (in the frame of H2020 project SMARTER n°772787). 653 $aEWE 653 $aProyecto SMARTER 653 $aResidual feed intake (RFI) 653 $aSHEEP 700 1 $aFERREIRA, G. 700 1 $aRAMOS, Z. 700 1 $aNAVAJAS, E. 700 1 $aCIAPPESONI, G.
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